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Cell Biology
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Cell Isolation Products
MojoSort(TM) Mouse CD3 T Cell Isolation Kit
MojoSort(TM) Mouse CD3 T Cell Isolation Kit from BioLegend
Be the first to write a review!
Citations:
(26)
Supplier Page
Supplier Page from
BioLegend for
MojoSort(TM) Mouse CD3 T Cell Isolation Kit
Description
MojoSort(TM) Mouse CD3 T Cell Isolation Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MojoSort(TM) Mouse CD3 T Cell Isolation Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
480024
Quantity
100 tests
Applications
Cell Sep - Neg
Format
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
Reactivity
Mouse
Target
CD3
Cell Type (standardized)
CD3+ T Cells
Conjugate/Tag/Label
MOJO
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BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (26)
(1)
IL-35 Stabilizes Treg Phenotype to Protect Cardiac Allografts in Mice
Transplantation
January 1, 2024
Ai Huang, Kewei Liu, Ziyi Yin, Jie Liu, Hongyan Wei, Shijie Xing, Yue Qu, Lei Huang, Liancheng Li, Chang Li,
et al.
retrieved from C57BL/6 WT
mice
. CD3+ T/CD8+ T cells were sorted using CD3 magnetic beads (
BioLegend
,
480024
)/
Dynabeads Untouched Mouse CD8 Cells Kit
(
Invitrogen
,
11417D
), and flow sorting was performed to ensure
(2)
TRAM deletion attenuates monocyte exhaustion and alleviates sepsis severity
Front Immunol
December 15, 2023
Jing Wang, Yajun Wu, RuiCi Lin, Yao Zhang, Liwu Li
coculture
Mouse spleen CD3+ T cells were isolates by using MojoSort Mouse CD3 T Cell Isolation Kit (
Biolegend
, #
480031
) resulting in >95% purity. Isolated
spleen
CD3+ T cells were labeled with 5 μM carboxyfluorescein succinimidyl
(3)
Functional divergence of a bacterial enzyme promotes healthy or acneic skin
Nat Commun
December 5, 2023
Irshad A. Hajam, Madhusudhanarao Katiki, Randall McNally, María Lázaro-Díez, Stacey Kolar, Avradip Chatterjee, Cesia Gonzalez, Mousumi Paulchakrabarti, Biswa Choudhury, J. R. Caldera,
et al.
by RBC lysis (Catalog number:
00-4300-54
,
eBioscience
™) and isolation of CD3+T cells (Catalog No.#
480031
,
Biolegend
, USA) by negative selection using MojoSort™ Mouse CD3 T Cell Isolation Kit as per the manufacturer’s
(4)
Human leukocyte antigen HLA-DR-expressing fibroblast-like synoviocytes are inducible antigen presenting cells that present autoantigens in Lyme arthritis
bioRxiv
December 1, 2023
Joseph R Rouse, Rebecca Danner, Amanda Wahhab, Michaela Pereckas, Mecaila E McClune, Allen C Steere, Klemen Strle, Brandon L Jutras, Robert B Lochhead
isolated from spleens of 6-to-8-week-old OT-II
mice
by MojoSort mouse CD3+ T cell isolation kit (
Biolegend
480024
). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5–10×105
(5)
Inhibition of SF3B1 improves the immune microenvironment through pyroptosis and synergizes with αPDL1 in ovarian cancer
Cell Death & Disease
November 27, 2023
Shourong Wang, Yao Liu, Huimin Xiao, Zhongshao Chen, Xiaohang Yang, Jingjing Yin, Yingwei Li, Cunzhong Yuan, Shi Yan, Gang Chen,
et al.
mouse
spleen
cells.
T cells
were separated by MojoSort Mouse CD3 T Cell Isolation Kit (
Biolegend
, cat#
480023
) from
mouse
spleen
cells. BMDM were separated from
mouse
bone marrow cells
by
incubation
with 10 ng/mL
(6)
mRNA‐based Vaccines Targeting the T‐cell Epitope‐rich Domain of Epstein Barr Virus Latent Proteins Elicit Robust Anti‐Tumor Immunity in Mice
Advanced Science
October 27, 2023
Ge‐Xin Zhao, Guo‐Long Bu, Gang‐Feng Liu, Xiang‐Wei Kong, Cong Sun, Zi‐Qian Li, Dan‐Ling Dai, Hai‐Xia Sun, Yin‐Feng Kang, Guo‐Kai Feng,
et al.
Separation Medium (Biotech, Shenzhen, China) and Mouse CD3 T Cell Isolation Kit (
BioLegend
, cat. #
480031
) following the manufacturer's protocol. Antibodies were purified from
mouse
serum using rProtein G Sepharose
(7)
Rap1 organizes lymphocyte front-back polarity via RhoA signaling and talin1
iScience
August 18, 2023
Yoshihiro Ueda, Koichiro Higasa, Yuji Kamioka, Naoyuki Kondo, Shunsuke Horitani, Yoshiki Ikeda, Wolfgang Bergmeier, Yoshinori Fukui, Tatsuo Kinashi
commercial
assays
,Critical commercial
assays
,, Mojosort Mouse CD3 T Cell Isolation Kit,
BioLegend
,Cat#
480031
Western Lightning Plus-
ECL
,PerkinElmer,Cat# NEL 104001EA ,,
Experimental models
: Cell lines,
Experimental models
(8)
Anti-phagocytosis-blocking repolarization-resistant membrane-fusogenic liposome (ARMFUL) for adoptive cell immunotherapy
Science advances
August 9, 2023
Zheng C, Zhong Q, Yi K, Kong H, Cao F, Zhuo C, Xu Y, Shi R, Ju E, Song W,
et al
.
from the
spleen
via magnetic bead separation (MojoSort Mouse CD8 T Cell Isolation Kit,
BioLegend
, no.
480031
), followed by culturing in the primary
T cell culture
system (iCell, no. PriMed-iCell-026). Further
(9)
Tumor-derived GDF-15 blocks LFA-1 dependent T cell recruitment and suppresses responses to anti-PD-1 treatment
Nature Communications
July 20, 2023
Markus Haake, Beatrice Haack, Tina Schäfer, Patrick N. Harter, Greta Mattavelli, Patrick Eiring, Neha Vashist, Florian Wedekink, Sabrina Genssler, Birgitt Fischer,
et al.
isolated from pooled spleens of BALB/c organ donor
mice
(MoJo
mouse
T cell
isolation kit;
Biolegend
#
480024
) and labeled with 5 µM carboxyfluorescein succinimidyl ester (
CFSE
) (
ThermoFisher
, #65-08-50-85). 5×106
(10)
Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow
Cell reports
June 27, 2023
Kamioka Y, Ueda Y, Kondo N, Tokuhiro K, Ikeda Y, Bergmeier W, Kinashi T.
commercial
assays
,Critical commercial
assays
,, Mojosort Mouse CD3 T cell Isolation Kit,
Biolegend
,Cat#
480031
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,,
Mouse
(11)
IL-10 production by granulocytes promotes Staphylococcus aureus craniotomy infection
Journal of Neuroinflammation
May 13, 2023
Gunjan Kak, Zachary Van Roy, Cortney E. Heim, Rachel W. Fallet, Wen Shi, Axel Roers, Bin Duan, Tammy Kielian
were pooled and subjected to CD3+ cell enrichment using a MojoSort™
Mouse
CD3 Selection Kit (Cat. #
480031
;
BioLegend
) per the manufacturer’s instructions. Purified CD3+ cells were treated immediately ex vivo
(12)
FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells
Nature communications
February 10, 2023
Zhao Q, Hu J, Kong L, Jiang S, Tian X, Wang J, Hashizume R, Jia Z, Fowlkes NW, Yan J,
et al
.
lymph nodes
of Balb/c, C57BL/6, FGL2−/−, and LAG3−/−
mice
and purified with a Mouse CD3 T cell Isolation Kit (
480031
;
BioLegend
). Human peripheral blood mononuclear cells (PBMCs) were isolated using
Ficoll-Paque PLUS
(13)
PD-1 and TIM-3 differentially regulate subsets of mouse IL-17A-producing γδ T cells
The Journal of experimental medicine
February 6, 2023
Edwards SC, Hedley A, Hoevenaar WHM, Wiesheu R, Glauner T, Kilbey A, Shaw R, Boufea K, Batada N, Hatano S,
et al
.
background were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (#
480031
;
Biolegend
) according to the manufacturer’s instructions. γδ
T cell
isolation was performed by TCRγδ
(14)
Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention Strategies
Blood cancer discovery
January 6, 2023
Flümann R, Hansen J, Pelzer BW, Nieper P, Lohmann T, Kisis I, Riet T, Kohlhas V, Nguyen PH, Peifer M,
et al
.
isolated from spleens of C57BL/6 wild-type
mice
using the Mouse CD3 T cell isolation kit (
BioLegend
; cat. #
480031
), cultured and activated as previously described (27). In brief,
T cells
were activated
in vitro
by
(15)
A microfluidics-based method for isolation and visualization of cells based on receptor-ligand interactions
PLOS ONE
October 6, 2022
Long Dao, Qingnan Zhao, Jiemiao Hu, Xueqing Xia, Qing Yang, Shulin Li
(Jackson, cat. No. 000664) Reagents MojoSort Mouse CD3 T cell Isolation Kit (
BioLegend
, cat. no.
480023
)
CellTracker Green BODIPY dye
(
Thermo Fisher Scientific
, cat. no.
C2102
)
CellTracker Red CMTPX dye
(
Thermo Fisher Scientific
(16)
Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
STAR Protocols
September 1, 2022
Xiaoqing Li, Peng Wu, Xiangjun Zeng, Qiulei Lang, Yu Lin, He Huang, Pengxu Qian
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Mouse
CD3
cell isolation
Kit,
BioLegend
,Cat.
480031
Pan
T-Cell
Isolation Kit,Miltenyi Biotec,Cat.130-0950139 E.Z.N.A.® Stool DNA Kit (200 preps),Omega,Cat#D4015-02 Phusion®
(17)
Inhibition of D-2HG leads to upregulation of a proinflammatory gene signature in a novel HLA-A2/HLA-DR1 transgenic mouse model of IDH1R132H-expressing glioma
Journal for ImmunoTherapy of Cancer
May 23, 2022
Pavlina Chuntova et al.
10-week-old dTG naïve
mice
were collected using the
Mouse
CD3
T-cell
Negative Selection Kit (
BioLegend
, #
480031
), labeled with 2 µM
CFSE
, washed, and added to all the wells of a round-bottom 96-well plate at 5×105
(18)
Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke
Stroke
October 1, 2021
Abdullah Al Mamun, Conelius Ngwa, Shaohua Qi, Pedram Honarpisheh, Saumil Datar, Romana Sharmeen, Yan Xu, Louise D. McCullough, Fudong Liu
C57BL/6
mouse
using the MojoSort™ Mouse CD3 T Cell Isolation Kit according to manufacture instruction (#
480024
BioLegend
). The purity of isolated CD3+ cells was confirmed by
flow cytometry
. The isolated cells were
(19)
Hypoxia-Driven Oncometabolite L-2HG Maintains Stemness-Differentiation Balance and Facilitates Immune Evasion in Pancreatic Cancer
Cancer Research
August 1, 2021
Vineet K. Gupta et al.
T cells
were isolated from
spleen
using MojoSort™ Mouse CD3 T Cell Isolation Kit (
Biolegend
Cat no;
480024
) as per the manufacturers protocol. Cells were labeled with
CFSE
(
ThermoFisher
cat no:
C34554
) as per
(20)
Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression
iScience
June 25, 2021
AbuEid M, McAllister DM, McOlash L, Harwig MC, Cheng G, Drouillard D, Boyle KA, Hardy M, Zielonka J, Johnson BD,
et al
.
commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, MOJO sort Kit,
Biolegend
,Cat#
480024
Dynabeads™
Mouse
T-Activator
CD3
/
CD28
,
Thermofisher Scientific
,Cat# 1456D Cell Trace
Violet
,
Thermofisher Scientific
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Reviews
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MojoSort(TM) Mouse CD3 T Cell Isolation Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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